ABSTRACT
OBJECTIVE@#To observe the changes of adenylate cyclase(AC) on cerebral regions related to morphine dependence in rats and investigate the relationship between the enzymological changes and the mechanism of morphine dependence.@*METHODS@#The technique of enzyme-histochemistry was used to detect the variations of AC of special seven cerebral regions including frontalis cortex, lenticula, corpus amygdaloideun, substantia nigra, hippocampus, periaqueductal gray and locus coerleus in morphine dependent rats. The enzymological changes were observed by optical microscope. Changes of gray degree of these cerebral regions were also observed by using the image analysis system.@*RESULTS@#Compared with those in control group, the contents of AC in morphine dependent groups were increased.@*CONCLUSION@#The contents of AC are increase in those regions. The mechanism of morphine dependence close related to the increasing of AC. The correlation of the mechanism of morphine dependence and up-regulation of AC/cAMP-PKA system is discussed.
Subject(s)
Animals , Female , Male , Rats , Adenylyl Cyclases/metabolism , Brain/pathology , Cerebral Cortex/enzymology , Disease Models, Animal , Hippocampus/enzymology , Morphine Dependence/pathology , Periaqueductal Gray/enzymology , Rats, Sprague-Dawley , Substance Withdrawal Syndrome/metabolism , Time FactorsABSTRACT
OBJECTIVE@#To investigate ultrastructural pathological changes of Heroin-Addicts.@*METHODS@#Heroin-Addicts' central nervous system, endocrine system, immune system and reproductive system in 4 cases are observed by using transmission electron microscope(TEM).@*RESULTS@#The changes of central nervous system are mitochondrion swelling, crista fragmentation and disappear. Endoplasmic reticulum dilation, nervous fibres and cell organelles reduction; mitochondrion swelling, Partial crista fragmentation and endoplasmic reticulum dilation are also found in endocrine system; Lymphocytes reduction, cytoplasm ingredient reduction and dead lymphocytes increase in immune system; in reproductive system, spermatogenic cells and cell organelles are reduced in the male and follicle disappeared in the female.@*CONCLUSION@#Ultra-structural pathological changes of heroin-addicts are presented acute, chronic oxygen deficiency degeneration and necrosis.